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Gibco™ DMEM/F-12, no phenol red

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ 21041025
Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: L-glutamine
Without: HEPES, Phenol Red
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature

Specifications
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts | |
1 X | |
DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
Sterile-filtered | |
No Phenol Red, No HEPES | |
Gibco™ | |
Room Temperature |
Animal Origin-free | |
Liquid | |
Standard Serum Supplementation | |
High Glucose, Glutamine, Sodium Pyruvate | |
cGMP-compliant under the ISO 13485 standard | |
500 mL | |
Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
2546549 | Certificate of Analysis | 29/01/2025 | 21041025 |
2451159 | Certificate of Analysis | 29/01/2025 | 21041025 |
2578369 | Certificate of Analysis | 29/01/2025 | 21041025 |
2477825 | Certificate of Analysis | 29/01/2025 | 21041025 |
2554973 | Certificate of Analysis | 29/01/2025 | 21041025 |
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For Research Use Only. Not for use in diagnostic procedures.