Learn More
Gibco™ DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
414.00 NOK
Specifications
Classification | Animal Origin-free |
---|---|
Concentration | 1 X |
Form | Liquid |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Sterility | Sterile-filtered |
Requires: Supplementation
Description
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
With: GlutaMAX, Phenol Red
Without: HEPES
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Animal Origin-free | |
Liquid | |
Sterile-filtered | |
No HEPES |
1 X | |
DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate | |
cGMP-compliant under the ISO 13485 standard |
Safety and Handling
missing translation for 'shelfLife' : 12 Months From Date of Manufacture
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.