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Thermo Scientific™ GeneBLAzer™ TRα-UAS-bla HEK 293T Assay Kit
TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1684
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Description
TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Specifications
| 9-cis-retinoic acid, TTNPB, CD437, AM 580 | |
| Cell-based beta lactamase reporter gene | |
| TR alpha-UAS-bla HEK 293T cells (K1260) Shipping: Dry Ice Storage:-80°C (liquid nitrogen) |
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| Fluorescence | |
| TR alpha-UAS-bla HEK 293T cells (K1260) | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Detection Assay Kit | |
| GeneBLAzer™ |
| AGN 193109 | |
| Dividing Cells | |
| Primary Agonist: All-trans-retinoic Acid; Antagonists: AGN 193109; Reporter: BLA (Beta-Lactamase) | |
| Nuclear Receptors | |
| All-trans-retinoic Acid | |
| 1 Plate | |
| THRA, TR alpha, NR1A1 | |
| HEK 293T | |
| Nuclear Receptors |
For Research Use Only. Not for use in diagnostic procedures.