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Invitrogen™ GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit
GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit
Brand: Invitrogen™ K1619
This item is not returnable.
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Description
The GeneBLAzer™ PAC1-CRE-bla CHO-K1 cells contain the human Adenylate Cyclase Activating Polypeptide 1 Receptor 1 (ADCYAP1R⁄PAC1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE). The PAC1-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, PAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_001118 | |
| Division-Arrested Cells | |
| Fluorescence | |
| Single Cell Clone Origin | |
| 8 x 106 cells | |
| ADCYAP1R1, PAC1 | |
| CHO-K1 | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| K1619 GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ PAC1 CHO-K1 DA cells • LiveBLAzer™-FRET B⁄G Loading Kit, 70μg • Solution D • GeneBLAzer™ PAC1 CHO-K1 DA Assay Protocol • LiveBLAzer™-FRET B⁄G Loading Protocol • Certificate of Analysis Storage: The GeneBLAzer™ PAC1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| ADCYAP1R1 | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.