missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ GeneBLAzer™ H2 HEK 293T DA Assay Kit

GeneBLAzer™ H2 HEK 293T DA (Division Arrested) cells and H2-CRE-bla HEK 293T cells contain the human Histamine Subtype 2 receptor (H2) stably integrated into the CellSensor™ CRE-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1307

Product Code. 10658134

  • 7560.00 NOK / Each
Estimated Shipment: 22-01-2025
to see stock.
Explore more special offers
Add to basket
This item is not returnable. View return policy

Description

Description

This cell line is available as dividing cells by requesting a quote for K1226 from dividingcells@invitrogen.com

GeneBLAzer™ H2 HEK 293T DA (Division Arrested) cells and H2-CRE-bla HEK 293T cells contain the human Histamine Subtype 2 receptor (H2) stably integrated into the CellSensor™ CRE-bla HEK 293T cell line. CellSensor™ CRE-bla HEK 293T cells (Cat. no.K1112) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both H2 HEK 293T DA cells and H2-CRE-bla HEK 293T cells are functionally validated for Z'-factor and EC50 concentrations of Histamine (Figure 1). In addition, H2-CRE-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Histamine 2 (H2) receptors have been implicated in a number of disorders, including peptic ulcer disease (PUD), gastroesophageal reflux disease (GERD), dyspepsia, and stress ulcer prophylaxis.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

BC054510.2
Division-Arrested Cells
Gene ID: 3274
GPCRs
HRH2
GeneBLAzer™
BLA (Beta-Lactamase)
Division-Arrested Cells Assay Kit
GeneBLAzer™
Cell-based beta lactamase reporter gene
GeneBLAzer™ H2 HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
• H2 HEK 293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D, 1 mL
• 2 protocols
• Certificate of Analysis

GeneBLAzer™ H2 HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
• H2 HEK 293T DA cells
• 1 protocol
• Certificate of Analysis

GeneBLAzer™ H2-CRE-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
• H2-CRE-bla HEK 293T cells
• 1 protocol
• Certificate of Analysis

Storage:
GeneBLAzer™ H2 HEK 293T DA Cells and H2-CRE-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.
Fluorescence
3274
Single Cell Clone Origin
1 Plate
HRH2, H2
293T (HEK)
GPCR
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.