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Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells
Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
Brand: Invitrogen™ T1030
Includes: Enzyme substrate and reaction buffer
Description
Gal-Screen homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.
- Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
- The kinetics of the glow-assay provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
- Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
- Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
- Assay sensitivity is 100 to 1000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
Specifications
- Compatible Cells: Mammalian Cells, Yeast Cells
- Detection Method: Chemiluminescent
- For Use With (Equipment): Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Substrate: Galacton-Star
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| Luminometer (Microplate) | |
| This kit contains the enzyme substrate and reaction buffer. Store the product at 4°C. |
|
| High-throughput Compatible | |
| End Point | |
| Galacton-Star™ | |
| Chemical Substrate | |
| Gal-Screen™, NovaBright™ | |
| 1000 Assays |
| Chemiluminescence | |
| β-Gal (lacZ) Assay | |
| Mammalian Cells, Yeast Cells | |
| Unconjugated | |
| Cell Cultures | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| Enzyme Assay | |
| Liquid |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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