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Thermo Scientific™ KpnI, HC (50 U/μL)

Thermo Scientific™ Fermentas KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion.

Brand: Thermo Scientific™ ER0523

2275.20 NOK valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 10785831

3250.00 NOK / 20000 units

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Description

Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI.

5'..G G T A C▵C...3'

3'..C▵C A T G G...5'

Conditions for 100% Activity:

1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 0.02% Triton X-100 and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer:

KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

Dam: never overlaps —no effect
Dcm: may overlap —no effect
CpG: may overlap —no effect
EcoKI: never overlaps —no effect
EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.

Specifications

Concentration	50 U/μL
Enzyme	Kpn I
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer	Unique Buffer (10X Buffer KpnI)
Optimal Reaction Temperature	37°C
Quantity	20,000 units
Research Category	Traditional Cloning

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Documents

Product Information

Manuals

User Guide: KpnI, HC, 50 U/uL, 20000U Version: Jan. 2015 File size: 66 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2813747	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0523
Lot Number2814514	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0523
Lot Number2864903	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0523
Lot Number2878189	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberER0523
Lot Number2934873	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberER0523

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