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Thermo Scientific™ GeneJET Plasmid Miniprep Kit
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Brand: Thermo Scientific™ K0502
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Description
GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.
Highlights
• Efficient—high yields of up to 20 μg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use
Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol
Specifications
| Spin Column | |
| Silica Column | |
| Bacteria | |
| Compatible (Vacuum manifold) | |
| GeneJET | |
| 50 Preps | |
| <14 min |
| Spin Column | |
| Mini | |
| PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid MiniPrep Kit | |
| Plasmid DNA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GeneJET Plasmid Miniprep Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : guanidine hydrochloride
Certificates
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Product Title
Thermo Scientific™ GeneJET Plasmid Miniprep Kit > For 50 preps
For Research Use Only. Not for use in diagnostic procedures.
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