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Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Brand: Thermo Scientific™ 21435
Description
Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Highlights:
- Water-soluble analog of NHS-LC-Biotin
- Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
- Negative charge of -SO3 group keeps biotinylation localized at the cell surface
- Creates an amide bond
Specifications
EZ-Link Sulfo-NHS-LC-Biotinylation Kit | |
10 reactions | |
1 to 10 mg | |
Water | |
Antibodies, Proteins |
Sulfo-NHS-LC-Biotinylation Kit | |
Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody • EZ-Link Sulfo-NHS-LC-Biotin, 25 mg • PBS Pack (makes 500 mL), 1 pack • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns • HABA Solution, 1 mL • Avidin, 10 mg Store biotin and avidin reagents at -20°C. Store remaining kit components at 4°C. |
|
EZ-Link™ | |
Biotin |
Safety and Handling
- Sulfo-NHS-LC-Biotinylation Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
- MIXTURE LIST-Contains : Sodium chloride, Sodium phosphate dibasic
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For Research Use Only. Not for use in diagnostic procedures.