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Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO
A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.
Brand: Thermo Scientific™ C20044
2130.10 NOK valid until 2025-03-29
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Description
- A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications
- The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules
- Once a protein or (bio)molecule is DBCO labeled, it can undergo a copper-free azide-alkyne cycloaddition reaction to form a stable triazole linkage with an azide labeled coupling partner
- Can be used to create diverse conjugates with the biorthogonal coupling between an alkyne and azide (supplied separately)
- The coupling or crosslinking procedure is typically done in two steps:
- First, a protein or thiol containing molecule is labeled with a DBCO group and excess crosslinker is removed
- Second, an azide containing moiety (prepared independently) is introduced into the same system as the DBCO moiety
- Labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst
- Reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
Specifications
| Azide-Sulfhydryl | |
| Maleimide-PEG4-DBCO | |
| Yes | |
| Upon receipt store desiccated at -20°C. | |
| Wet Ice | |
| Chemical Labeling | |
| Maleimide, DBCO | |
| Powder | |
| DMF, DMSO | |
| No |
| No | |
| 647.74 | |
| 29.7 Å | |
| Not determined | |
| EZ-Link™ No-Weigh™ | |
| Heterobifunctional | |
| Medium (10 to 30 Å) | |
| 10 x 1 mg | |
| Standard, Single-use | |
| Crosslinker |
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Certificates
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5 results found
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| ZL404502 | Certificate of Analysis | 31/01/2025 | C20044 |
| ZK403127 | Certificate of Analysis | 16/12/2024 | C20044 |
| ZK403127A | Certificate of Analysis | 16/12/2024 | C20044 |
| ZH402225 | Certificate of Analysis | 21/08/2024 | C20044 |
| ZG399923 | Certificate of Analysis | 08/08/2024 | C20044 |
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Product Title
Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO > 10 x 1 mg
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