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Gibco™ Expi293™ Expression Medium

Expi293™ Expression Medium designed for growth, transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.
Brand: Gibco™ A1435101
2320.11 NOK valid until 2025-03-21
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Description
Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.
- Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
- Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
- Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
- Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
- Does not contain phenol red
Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Specifications
Store in the dark at +2°C to +8°C | |
293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F | |
Animal Origin-free, Chemically-defined, Protein-free, Serum-free | |
Suspension Cell Culture | |
Expression Medium | |
Sterile | |
No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol | |
Bottle(s) | |
Calcium, Magnesium, Sodium Phosphate | |
Expi293™ | |
1 L |
CO2 | |
Embryonic Kidney Cells | |
1 X | |
Liquid | |
Serum-free | |
High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl | |
< 1 EU/mL | |
No antibiotics | |
275-295 mOsm/kg | |
Cell Culture Grade | |
Room Temperature |
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Safety and Handling
- Expi293 Expression Medium
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
- MIXTURE LIST-Contains : 4-Isopropoxybenzoicacid
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
3077955 | Certificate of Analysis | 31/01/2025 | A1435101,A1435104,A1435103 |
2796062 | Certificate of Analysis | 31/01/2025 | A1435101,A1435104,A1435103 |
2537618 | Certificate of Analysis | 29/01/2025 | A1435101,A1435104,A1435103 |
2537620 | Certificate of Analysis | 29/01/2025 | A1435101,A1435104,A1435103 |
2537622 | Certificate of Analysis | 29/01/2025 | A1435101,A1435104,A1435103 |
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For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.