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BD Biosciences™ DNA Reagent Kit (RUO/GMP)
Isolate and stain cell nuclei from surplus fresh or frozen solid tissue specimens or cell suspensions.
Brand: BD Biosciences™ 340242
This item is not returnable.
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Description
- Reagents for isolating and staining cell nuclei from surplus fresh or frozen solid tissue specimens or cell suspensions.
- Flow cytometric analysis of differentially stained normal and tumor cells is used for research in the identification of abnormal DNA stemlines and to estimate the DNA index (DI) and cell-cycle phase distributions of these stemlines.
Specifications
| When stored at −18°C, the reagents are stable until the expiration date shown on the BD Cycletest Plus DNA kit label when stored as directed. Do not use after the expiration date. Do not expose reagents to direct light during storage or incubation with cells. Do not heat reagents to 37°C. However, brief exposure to a 37°C water bath while thawing is acceptable.The thawed reagents are stable for 1 month when stored at 2°C–8°C. Do not refreeze after thawing. Alteration in the appearance of the reagents indicates instability or deterioration. Do not use if discoloration occurs or a precipitate forms. | |
| Buffer, trypsin solution, trypsin inhibitor/ribonuclease A solution, propidium iodide solution. | |
| 1 Kit: Buffer (3 x 50 mL), trypsin solution (10 mL), trypsin inhibitor/ribonuclease A solution (8 mL), propidium iodide solution (8 mL). |
| Suitable for 40 tests. | |
| BD FACScan or BD FACSort™ flow cytometer system equipped with a doublet discrimination module (DDM) or BD FACS Vantage™ flow cytometer equipped with pulse processing, and three-color fluorescence detection and two-parameter light-scatter detection |