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Thermo Fisher Scientific DDM/CHS Solution (10:1)

A ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity.

Brand:  Thermo Fisher Scientific A50942

Product Code. 17439292

  • 1896.00 NOK / Pack of 10

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Description

Description

  • A ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity
  • The stable pre-formulated solution contains a mixture of dodecyl-β-D-maltoside (DDM) (100 mg/mL) and cholesteryl hemisuccinate (CHS) (10 mg/mL) in deionized water with final concentrations of 10% and 1%, respectively
  • Typical working concentrations are 1% DDM/0.1% CHS for protein extraction and 0.1% DDM/0.01% CHS for protein purification

Features of DDM/CHS Solution include:

  • Ready-to-use liquid formulation
  • Mixture of nonionic detergent, DDM, with dual hydrophobic/hydrophilic properties and anionic detergent, CHS, that aids in the stabilization of large unilamellar vesicles
  • DDM/CHS micelles aid in solubilization of membrane proteins while preserving structure and function
  • Suitable for protein solubilization and stabilization for single particle cryo-EM analysis

Properties of DDM:

  • Molecular formula: C24H46O11
  • Molecular weight: 510.6 g/mol
  • Detergent class: Non-ionic
  • Micelle molecular weight: 50,000 g
  • Critical micelle concentration (CMC): 0.17 mM (0.009% w/v) in water; 0.12 mM (0.006% w/v) in 0.2 M NaCl

Properties of CHS:

  • Molecular formula: C31H50O4 · C4H11NO3
  • Molecular weight: 607.9 g/mol
  • Detergent: Ionizable anionic
  • Function: Stabilizes large unilamellar vesicles

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Liquid
10 x 1 mL
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