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Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit
CellROX™ Deep Red Flow Cytometry Assay Kit
Brand: Molecular Probes™ C10491
This item is not returnable.
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Description
The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).View a selection guide for all CellROX™ Reagents and Kits.
The CellROX™ Deep Red Flow Cytometry Assay Kit features:
The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
Specifications
| Mammalian Cells, Eukaryotic Cells | |
| Fluorescence | |
| Flow Cytometer | |
| CellROX™ Deep Red Reagent | |
| Tube(s) | |
| 100 Assays | |
| DMSO (Dimethylsulfoxide) |
| Contains 1 vial CellROX™ Deep Red Reagent (25 μL), 1 vial SYTOX™ Blue Dead Cell Stain (100 μL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 μL, 70% solution in water), and 1 vial DMSO (200 μL). Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive. |
|
| Flow Cytometry | |
| Reagent | |
| CellROX™ Deep Red: 644⁄665, SYTOX™ blue: 444⁄480 | |
| CellROX™ | |
| Wet Ice |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- CellROX Deep Red Flow Cytometry Assay Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- Serious eye damage/eye irritation Category 1
- Flammable liquid Category 3
- Germ cell mutagenicity Category 2
- Organic peroxide Type E & F
- Skin corrosion/irritation Category 1 B
- Skin sensitiser Category 1
Hazard Statement
- H226-Flammable liquid and vapour.
- H242-Heating may cause a fire.
- H302-Harmful if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H331-Toxic if inhaled.
- H341-Suspected of causing genetic defects.
- H411-Toxic to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P273-Avoid release to the environment.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P370+P378-In case of fire: Use water/water spray/water jet/carbon dioxide/sand/foam/alcoholresistant foam/chemical powder to extinguish.
Supplemental information
- MIXTURE LIST-Contains : tert-Butyl hydroperoxide
For Research Use Only. Not for use in diagnostic procedures.