CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio129c (129c)), APC, eBioscience™, Invitrogen™

Description

Description: The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predomitly expressed by IPCs in the naive mouse which represent sa very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8. The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs. Applications Reported: This eBio129c (129c) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio129c (129c) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.