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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Brand: Molecular Probes™ A23210

Product Code. 10043102

6430.00 NOK / 5mL

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Description

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

Specifications

Color	Green
Detection Method	Fluorescence
Excitation/Emission	490/525
For Use With (Application)	Flow Cytometry
Label or Dye	Alexa Fluor™ 488
Product Line	APO-BrdU™
Species	Mouse
Label Type	Alexa Fluor Dyes

Content And Storage	Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL). Store as directed.
Format	Tube
Flow Cytometer Laser Lines	488
For Use With (Equipment)	Fluorescence Microscope, Flow Cytometer
Product Type	TUNEL Assay Kit
Quantity	60 Assays
Target	BrdU

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Safety and Handling

Product Identifier

APO-BrdU TUNEL Assay Kit, with Alexa Fluor 488 Anti-BrdU

Signal Word

Danger

Hazard Category

Serious eye damage/eye irritation Category 2
Flammable liquid Category 2

Hazard Statement

H225-Highly flammable liquid and vapour.
H319-Causes serious eye irritation.

Precautionary Statement

P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233-Keep container tightly closed.
P240-Ground/bond container and receiving equipment.
P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
P242-Use only non-sparking tools.
P243-Take precautionary measures against static discharge.
P264-Wash hands thoroughly after handling.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313-If eye irritation persists: Get medical advice/attention.
P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P403+P235-Store in a well-ventilated place. Keep cool.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : ethanol

Documents

Product Information

Manuals

User Guide: APO-BrdU TUNEL Assay Kit Version: B.0 File size: 113 kB

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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU > 60 assays

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