BD Biosciences™ Anti-Human γ2a FITC/γ1 PE/CD4 PerCP-Cy™5.5/CD3 APC (RUO/GMP)

Description

• Clone SK7 (also known as Leu-4) X39 X40 SK3 (also known as Leu3a) (RUO (GMP))
• γ_2a (IgG_2a), clone X39, and γ₁ (IgG₁), clone X40, are both derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with KLH.
• CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.
• CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.
• Both γ_2a (IgG_2a) and γ₁ (IgG₁) react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.
• The CD4 antibody recognizes an antigen, with a molecular weight of 55-kilodalton (kDa) that is present on T-helper/inducer lymphocytes and monocytes.
• CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).
• Contents:
• Specificity: CD3, Clone: SK7 (also known as Leu4), Format APC, Isotype IgG₁ K, Entrez Gen ID: 916
• Specificity: Mouse γ_2z, Clone: X39, Format FITC, Isotype IgG_2a K, Entrez Gen ID: 380793
• Clone: X40, Format PE, Isotype IgG₁ K
• Specificity: CD4, Clone: SK3 (also known as Leu-3a), Format PerCP-CY™5.5, Isotype IgG₁ K, Entrez Gen ID: 920
• Research applications include the following:
  • Studies of T-cell responses to antigens such as cytomegalovirus (CMV), human immunodeficiency virus (HIV), herpes viruses, and tumor antigens.