Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.
Brand: Thermo Scientific™ 20475
Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns.
The resins and kits use activated beaded agarose and a robust coupling chemistry to immobilize proteins and other ligands through primary amines (-NH2) to the resin. Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride). The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20mg of immobilized protein per milliliter of agarose beads.
Highlights:
AminoLink Plus Coupling Resin–aldehyde-activated crosslinked 4% beaded agarose
Ideal for antibodies and other proteins– immobilize molecules via primary amines (-NH2)
Flexible coupling conditions– efficient ( >85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (borate, pH 10) coupling protocols provided
Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions
Convenient kits and product sizes – choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin; bulk quantities available for manufacturing applications
Includes:
Kits contain AminoLink Plus Columns, neutral and enhanced coupling buffers (Part No. 44894 only), quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories
| 25 to 100 μg Protein Immobilization per Column | |
| Sufficient For: Immobilizing 25 to 100 μg of protein per column; Includes buffers for 20 affinity purifications • AminoLink Plus Spin Columns, 0.1 mL, 10 columns • PBS Pack (makes 500 mL), 1 pack • Sodium Cyanoborohydride Solution, 5M, 0.5 mL • Quenching Buffer, 60 mL • Wash Solution, 25 mL • Elution Buffer, 50 mL • Collection Tubes, 200 tubes Store at 4°C. |
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| 10 Column Kit | |
| Immobilization Kit | |
| 25 to 100 μg of Protein per Column | |
| Affinity, Agarose Resin |
| Multi-component kit with 10 x 0.1mL resin columns | |
| AminoLink™ | |
| Antibody, Proteins through Primary Amine | |
| Aldehyde | |
| Kit, Microcentrifuge, Spin Column |
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For Research Use Only. Not for use in diagnostic procedures.
