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Amersham™ CyDye™ 700 Goat-Anti-Mouse Secondary Antibody, Cytiva
Amersham™ CyDye™ NIR-labeled secondary antibodies are designed for quantitative fluorescent Western blotting.
Brand: Life Science products Cytiva 29360784
Description
Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents. Different fluorophores are available for signal detection at either visible light or near-infrared (NIR) wavelengths.
Amersham CyDye 700 and 800 secondary antibodies are labeled with NIR fluorophores that emit light at wavelengths of 700 nm or 800 nm. By using Amersham CyDye 700 and 800 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.
Key benefits:
- Easy handling and a strong signal-to-noise ratio yield reproducible results
- Multiplexing analysis of more than one target protein on a single blot increases process efficiency
- Total protein normalization (TPN) generates reliable and accurate quantitation
- Stable signals allow membranes to be stored and rescanned for future studies
Specifications
Western Blot | |
0.1 mg | |
2°C to 8°C, protected from light |
CyDye 700 | |
Secondary |
Safety and Handling
- Amersham CyDye 700nm goat antimouse (0.1mg)
Hazard Category
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P273-Avoid release to the environment.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : sodium azide
- UNKNOWN ACUTE-50.9 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 5.9% of components with unknown hazards to the aquatic environment.