Invitrogen™ pMIR-REPORT™ miRNA Expression Reporter Vector System

Description

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR miRNA Inhibitor Molecules, respectively.

• Contains two mammalian expression vectors: one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency
• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Vector-Based RNAi, miRNA and Non-Coding RNA Analysis, miRNA Functional Analysis

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