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Description
mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.
- RNA Yield: 5-25μg
- Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
- Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
Specifications
| SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. | |
| Kit | |
| In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products | |
| Ambion™, mMESSAGE mMACHINE™ | |
| 25 reactions | |
| Dry Ice | |
| PCR Products, Linearized DNA plasmid |
| Synthetic mRNA, mRNA | |
| mRNA, Synthetic mRNA | |
| 25 Reactions | |
| SP6 In Vitro Transcription kit | |
| SP6 | |
| 15-25 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Message Machine
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Reproductive toxicity Category 1B
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : formamide, lithium chloride
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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