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Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Brand:  Invitrogen™ AM1700

7121.20 NOK valid until 2025-03-28
Use promo code "24107" to get your promotional price.

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Product Code. 10554445

  • 9820.00 NOK / Each

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Includes: Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Requires : SuperTaq Thermostable Taq DNA Polymerase sold separately. For optimal amplification of fragments ≥1 kb, use SuperTaq-Plus.
This item is not returnable. View return policy

Description

Description

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

  • From RNA to PCR product in less than a day
  • Yields single, specific product from rare transcripts
  • Selects 5' and/or 3' ends of true messages
  • Efficient—
  • Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
  • Final product size: 7kb or less
  • Optimal reaction temperature: 42°C
  • Sample: RNA

  • Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
  • Improvement to basic RACE protocol
  • Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

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Shipping Condition: Dry ice

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Protocol: FirstChoice® RLM-RACE Kit (English ) Version: 09 Sep 2010 File size: 659.9 kB
FirstChoice® RLM-RACE Kit Instruction Manual Version: 0803C File size: 659.9 kB
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Lot Number Certificate Type Date Catalog Number
Lot Number2849503 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog NumberAM1700
Lot Number2808152 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM1700
Lot Number2824030 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM1700
Lot Number2824281 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM1700
Lot Number2824084 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberAM1700
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For Research Use Only. Not for use in diagnostic procedures.