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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Brand: Invitrogen™ A25602
41267.50 NOK valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Description
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
This new addition to the family includes a TaqMan™ 1-Step RT-PCR master mix. The single-tube format of the RT-PCR step streamlines the 'sample to answer' workflow more than ever. Combining the simple Cells-to- CT™ lysis technology with the fast cycling capability of the 1-step RT-PCR master mix allows quicker time to results with limited sample handling.
The Cells-to-CT™ 1-Step TaqMan™ Kit is:
- Complete—optimized workflow includes cell lysis reagents with gDNA removal and TaqMan™ 1-Step RT-PCR Master Mix
- Faster than ever—7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for ∼35 minute sample-to-answer workflow
- Simple—10 steps total (sample prep and real-time RT-PCR combined)
- Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
- Convenient—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs
Note: TaqMan™ primer/probe sets are sold separately.
Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR
Whether you are using plates or tubes, the Cells-to-CT™ 1-Step TaqMan™ Kit uses a simple 7-minute sample preparation procedure. The lysis technology is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysate is now ready to analyze on your real-time PCR instrument. Simply add it to a real-time RT-PCR master mix containing the TaqMan™ primer/probe sets of interest. Alternatively, the lysate can be stored at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.
A Complete, Validated Solution
All components of the Cells-to-CT™ 1-Step TaqMan™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes. For added quality assurance, the Cells-to-CT™ 1-Step TaqMan™ Kit has been validated with TaqMan™ Gene Expression assays and shows performance equivalent to that obtained with purified RNA.
Accessory Product
The TaqMan™ Cells-to-CT™ Control Kit (Cat. No. 4386995) was designed for use with TaqMan™ Cells-to-CT™ products. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan™ Gene Expression assay to monitor the effects of PCR inhibition.
Specifications
Lysis Solution, 22 mL, store at 2°C to 8°C, DNase I, 220 μL, store at -5 to -30°C, Stop Solution, 2 x 1 mL, store at -5 to -30°C, TaqMan™ 1-step qRT-PCR Mix, 10 mL, store at -5 to -30°C, | |
Tube | |
1-step RT-qPCR | |
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system | |
Cells-to-CT™, TaqMan™ | |
400 preps | |
400 Reactions | |
Mammalian Cells | |
22 mL |
TaqMan | |
High | |
7 min. | |
Fewer resources used and less waste, Less hazardous | |
1-Step qPCR kit | |
Gene Expression | |
Cell Lysis | |
10-100,000 Cells |
Safety and Handling
- Cells-to-CT 1-Step TaqMan Kit
Signal Word
- Warning
Hazard Category
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P264-Wash thoroughly after handling.
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : Glycerin, Ethylene oxide (impurity), Glycerin, Dimethyl sulfoxide, Ethylene glycol, Tris (hydroxymet
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For Research Use Only. Not for use in diagnostic procedures.