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Abnova™ 293T Whole Cell Lysate (Denatured)
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Quantity:
100 μL
Unit Size:
100µL
Specifications
Specifications
Cell Line | 293T |
For Use With (Application) | Western Blot |
Tissue | Kidney |
Description | Whole cell lysate (denatured) |
Lysis Buffer | 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue). |
Preparation Method | Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly. |
Quality Control Testing | 12.5% SDS-PAGE Stained with Coomassie Blue. |
Quantity | 100 μL |
Storage Buffer | In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue). |
Host Species | Human |
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Safety and Handling

Product Identifier
- 100UL 293T whole cell lysate (denatured)
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P102-Keep out of reach of children.
- P233-Keep container tightly closed.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 2-mercaptoethanol
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