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Cytiva 2-D Quant Kit
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. Cytiva Life Sciences™ 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
Brand: Cytiva 80-6483-56
Includes: Precipitant, 250mL; Co-precipitant, 250mL; Copper solution, 50mL; Color reagent A, 2 x 250mL; Color reagent B, 5mL; BSA standard (2mg/mL), 5mL
Description
Designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques. Works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein.
- Works in the presence of 2% SDS, 1% DTT, 8M urea, 2M thiourea, 4% CHAPS, 2% Pharmalyte™, and 2% IPG Buffer
- Quantitatively precipitates proteins while leaving interfering substances behind
- Linear response in the range of 0 to 50μg protein, with sample volumes of 1 to 50μL
- Assay time: one hour
2-D electrophoresis, SDS-PAGE, IEF
Specifications
| 2D Electrophoresis, SDS-PAGE, IEF | |
| 1 to 50μL | |
| 2D Quant kit |
| 250mL Precipitant, 250mL Co Precipitant, 50mL Copper Solution, 2 x 250mL Color reagent A, 5mL Color reagent B, 5mL BSA standard (2mg/mL) | |
| 4° to 8°C | |
| 500 Assays |
Safety and Handling
Supplemental information
- MIXTURE LIST-Contains : sodium carbonate, Cholan-24-oic acid, 3,12-dihydroxy-, monosodiumsalt, monohydrate, (3.alpha., 5.be
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