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Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Brand: Thermo Scientific™ 34018
This item is not returnable.
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Description
Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.
TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.Highlights:
HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media
Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates
Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use
Complete – does not require other buffers or addition of hydrogen peroxide
Specifications
| Substrate | |
| Liquid | |
| 250 mL | |
| Horseradish Peroxidase |
| Colorimetric | |
| 1-Step™ | |
| Horseradish Peroxidase | |
| Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 1-Step TMB-Blotting, 250 mL
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Specific target organ toxicity Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H371-May cause damage to organs.
Precautionary Statement
- P270-Do not eat, drink or smoke when using this product.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
Supplemental information
- MIXTURE LIST-Contains : Methyl alcohol
For Research Use Only. Not for use in diagnostic procedures.