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Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution
1-Step™ Chloronaphthol Substrate Solution
Brand: Thermo Scientific™ 34012
Description
Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.
Features of Chloronaphthol Substrates:
Highlights of Pierce CN Solution

Specifications
Substrate | |
Liquid | |
250 mL | |
Horseradish Peroxidase |
Colorimetric | |
1-Step™ | |
Horseradish Peroxidase | |
Store at 4°C. |
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Safety and Handling
- 1-Step Chloronaphthol Substrate Solution
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Specific target organ toxicity Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H371-May cause damage to organs.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : methanol
Certificates
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For Research Use Only. Not for use in diagnostic procedures.