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Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
Particle Size | 45 to 165 μm |
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pH Range | 4 to 13 |
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
Particle Size | 60 to 160 μm |
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Ligand Type | Talon Superflow Cobalt-IMAC |
Flow Rate | 1 to 4 mL/min |
Matrix | 6% Cross-Linked Agarose |
Storage Buffer | 20% Ethanol |
pH Range | 3 to 12 |
Product Type | HiTrap Talon crude prepacked Column |
Material | Polypropylene |
Storage Requirements | 4°C to 8°C |
For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
Max. Pressure | 3 bar |
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
Particle Size | 34 μm |
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Ligand Type | Strep-Tactin XT |
Density (Metric) | Approx. 5 mg/mL resin |
Matrix | Rigid, cross-linked agarose, spherical |
Stability | Stable in all commonly used aqueous buffers |
pH Range | 6 to 10 pH |
Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
Storage Requirements | 2 to 8°C in 20% ethanol |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva Capto™ S Chromatography Media
Designed for capture and intermediate purification of proteins from large feed volumes. Cytiva CaptoTM S Chromatography Media is a strong cation exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers.
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: DEAE
Used for preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ Sephaorose™ Fast Flow Anion Exchange Media: DEAE ensures low levels of non-specific binding.
Cytiva MabSelect™ Chromatography Media
Protein A affinity medium designed for capturing MAbs from large sample volumes
Includes | Certificate of Analysis |
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Particle Size | 85 μm |
Ligand Type | Recombinant Protein A (E. coli) |
Flow Rate | <500 cm/Hoμr |
Matrix | Highly Cross-Linked Agarose |
pH Range | 3 to 10 |
Product Type | MabSelect Media |
Binding Capacity | 30 mg Human lgG/mL (at 2.4 min. residence time) |
Height (English) Bed | 7.87 in. |
Storage Requirements | 2°C to 8°C |
For Use With (Application) | For Industrial AppliCatIons and Well-Established in Approved Processes |
Height (Metric) Bed | 20 cm |
Max. Pressure | <200 kPa |
Cytiva rProtein A Sepharose™ Fast Flow Affinity Media
Designed for purification of monoclonal and polyclonal antibodies at labs and process scales
Particle Size | 60 to 165 μm |
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Ligand Type | Recombinant Protein A (E. coli) |
Flow Rate | 150 to 250 cm/Hoμr |
Matrix | 4% Cross-Linked Agarose |
Stability | Stable in aqueous Buffers commonly used in Protein A Chromatography |
Storage Buffer | 20% Ethanol |
pH Range | 2 to 9 |
Product Type | rProtein A Sepharose Fast Flow Media |
Height (English) Bed | 9.84 in. |
Storage Requirements | 2°C to 8°C |
For Use With (Application) | For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales |
Height (Metric) Bed | 25 cm |
Max. Pressure | 100 kPa |
Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media
Use for coupling amino-containing smaller proteins and peptides. Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.
Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media
Used for capture and intermediate purification requiring low to medium hydrophobicity
Ligand Type | Phenyl |
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Matrix | 6% Cross-Linked Agarose |
pH Range | 3 to 13 |
For Use With (Application) | For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity |
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva SP Sepharose™ High Performance Media
Use for high intermediate and polishing steps in downstream processing. Cytiva Life Sciences™ SP Sepharose™ High Performance Media is composed of crosslinked agarose beads.
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
Particle Size | 60 to 160 μm |
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Ligand Type | Talon Superflow Cobalt-IMAC |
Matrix | 6% Cross-Linked Agarose |
Storage Buffer | 20% Ethanol |
pH Range | 3 to 12 |
Product Type | Talon Superflow Media |
Binding Capacity | Up to 20 mg Histidine-tagged Protein |
Storage Requirements | 4°C to 8°C |
For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media
Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.
Particle Size | 90 μm |
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Ligand Type | Native Protein A (S. aureus) |
Flow Rate | 150 to 250 cm/Hoμr |
Matrix | 4% Cross-Linked Agarose |
Stability | No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol |
Storage Buffer | 20% Ethanol |
Product Type | nProtein A Sepharose 4 Fast Flow Media |
Binding Capacity | >35 mg Human IgG/mL |
Height (English) Bed | 9.84 in. |
Storage Requirements | 2°C to 8°C |
Height (Metric) Bed | 25 cm |
Max. Pressure | 100 kPa |