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Cytiva NAP™ Columns, NAP-25
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-25 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva NAP™ Columns, NAP-10
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-10 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Thermo Scientific™ GeneJET Plasmid Miniprep Kit
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MiniPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Mini |
High-throughput Compatibility | Compatible (Vacuum manifold) |
For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
Test Time | <14 min |
Product Line | GeneJET |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Phasemaker™ Phasemaker™ Tubes
Invitrogen™ Phasemaker™ Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol™ mix from the organic phase underneath, making it very easy to pipette off the upper phase containing the RNA.
Thermo Scientific™ GeneJET Plasmid Midiprep Kit
Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MidiPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Midi |
Concentration | 400 ng/μL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
Test Time | 60 min. |
Product Line | GeneJET |
Prep Scale | 100-200 μg (Medium-Scale) Plasmid DNA |
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MaxiPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Maxi |
Concentration | 400 ng/μL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
Test Time | 60 min. |
Product Line | GeneJET |
Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
Invitrogen™ PureLink™ RNA Mini Kit
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA |
Sample Type | Bacteria,Blood,Cells,Plant,Tissue,Yeast,Enzymatic Reactions |
Isolation Technology | Silica Spin Column |
Purification Time | 20 min. (After sample homogenization) |
Yield | Up to 350 μg |
Scale | Mini |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Cytiva NAP™ Columns, NAP-5
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-5 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Phasemaker™ TRIzol™ Reagent and Phasemaker™ Tubes Complete System
This system includes both TRIzol™ Reagent and Invitrogen™ Phasemaker™ Tubes.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA |
Sample Type | Bacteria,Cells,Plants,Tissue,Yeast |
Isolation Technology | Organic Extraction |
Starting Material Amount | Bacteria: ≤107 cells Cells: ≤107 Plant: ≤107 cells, 100 mg Tissue: ≤100 mg Yeast: ≤107 cells |
Purification Time | 1 hr. |
Yield | Cells: ≤15 μg Tissue: ≤73 μg |
Elution Volume | 20 to 50 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Invitrogen™ TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System
This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes.
Phasemaker™ TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System
This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA |
Sample Type | Liquid Samples (e.g. Serum) |
Isolation Technology | Organic Extraction |
Starting Material Amount | 0.25 mL per 0.75 mL of TRIzol™ LS Reagent |
Purification Time | 1 hr. |
Yield | Tissue: ≤10 μg per mg Cells: ≤15 μg per 106 cells |
Elution Volume | 20 to 50 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Cytiva MicroSpin™ G-25 Columns
Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.