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Protein Purification

Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (262)
Gel Filtration Media and Columns (93)
Affinity Gels (34)
Spin Columns and Gravity-flow Columns (22)
Biotin-Binding Affinity Resins and Beads (6)
Magnetic Affinity Separation Particles (5)
Protease Inhibitors and Phosphatase Inhibitors (1)

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1 – 15 of 219 results
1

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
2

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
3

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

Promotions are available
EDGE
4

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Promotions are available
Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
5

Cytiva Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

Promotions are available
EDGE
6

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

Promotions are available
7

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
8

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Promotions are available
9

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Promotions are available
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
10

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Promotions are available
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
11

Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
12

Cytiva Glutathione Sepharose™ 4B Media

Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.

Promotions are available
Particle Size 90 μm
pH Range 4 to 13
Material Glutathione Sepharose 4 B
13

Cytiva MBPTrap™ HP Prepacked Columns

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 2° to 8°C
Bed Volume 1mL
pH Range >7
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Purification of MBP tagged Proteins
Max. Pressure 3 bar
Particle Size 34μm
Ligand Type Dextrin
Product Type Dextrin Sepharose HP prepacked column
Support Type 6% Highly Cross-linked Agarose
Height (English) 0.98 in.
14

Cytiva His Mag Sepharose™ Ni Magnetic Beads

Designed for efficient, small-scale purification/screening of histidine-tagged proteins from different sources Cytiva Lifescience™ His Mag Sepharose™ Ni Magnetic Beads enable high-capacity small-scale purification.

Binding Property 50 mg Histidine-tagged protein/mL medium