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Enzymes and Inhibitors

Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (8)
Restriction Enzyme Buffers (1)
Modifying Enzymes (1)

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1 – 10 of 10 results
1

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

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Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme BpiI (BbsI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X Buffer G
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
2

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Eco31I
Methylation Sensitivity CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
3

Thermo Scientific™ OliI (AleI) (10 U/μL)

The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme OliI (AleI)
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer R
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
4

Thermo Scientific™ Esp3I (BsmBI) (10 U/μL)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Esp3I (BsmBI)
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X Buffer Tango
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
5

Thermo Scientific™ MssI (PmeI) (5 U/μL)

The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme MssI (PmeI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer B
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 5 U/μL
6

Thermo Scientific™ RNase T1 (1000 U/μL)

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Promotions are available
Product Type RNase T1
Enzyme RNase
Concentration 1000 U/μL
7

Thermo Scientific™ Tango Buffer (10X)

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.

Promotions are available
8

Thermo Scientific™ XhoI (10 U/μL)

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).

Concentration 10 U/μL
9

Thermo Scientific™ FastDigest Green Buffer (10X)

Proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes.

Promotions are available
10

Thermo Scientific™ SmaI (10 U/μL)

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).

Concentration 10 U/μL