Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Thermo Scientific™ SuperBlock™ Blocking Buffer
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
Promotions are available
Thermo Scientific™ StartingBlock™ Blocking Buffer
StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.
Promotions are available
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
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Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Promotions are available
Thermo Scientific™ Fish Serum Blocking Buffer
Thermo Scientific Fish Serum Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in immunohistochemistry (IHC) and other detection methods involving mammalian samples.
Promotions are available
Medicago Phosphate Buffered Saline Tablets with Tween™ 20, pH 7.4
Supplied as exactly pre-weighed tablets with a final buffer volume of 500 or 1000ml. Medicago Phosphate Buffered Saline Tablets with Tween™ 20, pH 7.4 is dissolved in deionized water to yield a solution of sodium chloride, potassium chloride, Tween™ 20 and phosphate buffer.
Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
Promotions are available
Thermo Scientific™ SuperBlock™ Blocking Buffer, blotting
Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.
Promotions are available
Thermo Scientific™ Borate-buffered saline (5X)
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Thermo Scientific™ Pierce™ Fast Blocking Buffer
Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.
Promotions are available
Thermo Scientific Chemicals Tween 20 washing buffer, 0.5% in PBS (10X)
Promotions are available
Thermo Scientific™ Pierce™ Fast Wash Buffer, 10X
Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.
Promotions are available
Thermo Scientific™ Pierce™ Clear Milk Blocking Buffer (10X)
Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.
Promotions are available