HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

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Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

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Specifications

Pore Size	120 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Pricing and Availability
Specifications

Carbon Load	11%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L1

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	15%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	350 m²/g
USP Type	L1

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	8%
Stationary Phase	Proprietary
Temperature	70°C
Phase	HILIC
For Use With	LC/MS
Endcapped	Yes
Column Type	HILIC
pH	2 to 8
Surface Area	300 m²/g
Product Line	Acclaim

Hamilton™ PRP-X100 Anion Exchange HPLC Columns: Stainless Steel

Polymeric column packing for separation of inorganic and organic anions from 10 to 500 ppm

Pricing and Availability
Specifications

Pore Size	100 Å
Type	Column
Stationary Phase	PRP-X100
Temperature	5°C to 60°C
Phase	Ion Exchange
Packing Material	PSDVB/Trimethylammonium
Column Type	Ion Exchange
Mobile Phase Compatibility	pH 1 to 13, 0 to 100% aqueous, organic modifier
pH	1 to 13
Material	Stainless Steel
Max. Pressure	5000 psi (345 bar)
USP Type	L47

Thermo Scientific™ ProPac™ 3R SAX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SAX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and AAVs.

Pricing and Availability
Specifications

Pore Size	Nonporous
Particle Size	3 μm
Stationary Phase	Proprietary
Temperature	Ambient to 60°C
Phase	Strong Anion Exchange
Packing Material	Monodisperse Polymer Resin
Column Type	Ion Exchange
pH	2 to 12
Technique	Bio-LC
Max. Pressure	4500 psi (310 bar)
Product Line	ProPac
Column Format	Analytical Column

Thermo Scientific™ HyperREZ™ XP Carbohydrate H+ HPLC Columns

Manufactured with a proprietary process and subjected to stringent quality control procedures, these LC Columns are used for the appropriate counter-ion to meet application requirements.

Pricing and Availability
Specifications

Particle Size	8 μm
Phase	Ligand Exchange
For Use With	LC/MS
Packing Material	Spherical, Microporous Polymer
pH	1 to 11
Max. Pressure	725 psi (50 bar)

Thermo Scientific™ HyPURITY™ ADVANCE™ HPLC Columns, 5μm Particle Size

Use this specialty C8 column for separating compounds showing poor peak shape with traditional alkyl bonded phases.

Pricing and Availability
Specifications

Particle Size	5 μm
Carbon Load	9%
Stationary Phase	C8
Phase	Reversed Phase
Packing Material	Silica
Column Type	Reversed Phase
Surface Area	200 m²/g
USP Type	L7

Thermo Scientific™ Hypersil GOLD™ C8 HPLC Columns

Have analytes with medium hydrophobicity or need a less hydrophobic phase to obtain optimum retention? These columns provide outstanding sensitivity and excellent peak shape.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	8%
Stationary Phase	C8
Temperature	60°C
Column Type	Reversed Phase
pH	2 to 9
Surface Area	220 m²/g
USP Type	L7

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Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC Columns

Obtain exceptional peak shape and high resolution for fast UHPLC high-pressure, high-throughput applications using generic gradients on Vanquish UHPLC and LC/MS systems.

Pricing and Availability
Specifications

Pore Size	175 Å
Stationary Phase	C18 Selectivity
Temperature	60°C
Phase	Reversed Phase
For Use With	LC/MS
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	220 m²/g
Column Format	Analytical Column
Particle Size	1.9 μm
Carbon Load	11%
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric)	2.1 mm
pH	1 to 11
Technique	Bio-LC
Product Line	Hypersil GOLD
USP Type	L1

Macherey-Nagel™ Nucleosil™ 100-5 Nautilus Analytical HPLC Standard EC Column

Pricing and Availability
Specifications

Pore Size	100 Å
Solvents	Acetonitrile/water
For Use With	HPLC
Endcapped	Yes
Quantity	1 Ea.
Crosslinking	Octadecyl
Column Chemistry	Silica
Column Format	Analytical Column
Particle Size	5 μm
Type	Analytical EC Column
Carbon Load	16%
Packing Material	Silica
Mobile Phase Compatibility	Acetonitrile/water
pH	2 to 8

Thermo Scientific™ ProPac™ Elite WCX HPLC Column

This column provides consistent separations of therapeutic proteins including monoclonal antibodies, antibody drug conjugates, biosimilars and charge variants.

Pricing and Availability
Specifications

Pore Size	Nonporous
Stationary Phase	Carboxylate
Temperature	10°C to 60°C
Phase	Ion Exchange
Column Type	Ion Exchange
Green Features	Responsibly packaged
Max. Pressure	4500 psi (310 bar)
Column Format	Analytical Column
Particle Size	5 μm
Packing Material	Nonporous DVB Particles with Hydrophilic Coating
Mobile Phase Compatibility	Recommended: Goods Buffers and NaCl. Minimum 20 mM salt required. Do not rinse with pure DI H₂O
pH	2 to 12
Technique	Bio-LC
Product Line	ProPac

Thermo Scientific™ Hypersil™ PREP ODS C18 HPLC Columns

Perfect for routine workflows, our Hypersil PREP ODS preparative LC column is a general purpose C18 used in a broad range of USP L1 applications.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous Silica
Column Type	Reversed Phase
pH	2 to 8
Surface Area	195 m²/g
USP Type	L1

Thermo Scientific™ Hypersil GOLD™ SAX HPLC Columns

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil™ GOLD SAX LC Columns.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	2.5%
Stationary Phase	Quaternary Amine
Temperature	60°C
Phase	Ion Exchange
Column Type	Ion Exchange
pH	2 to 8
Surface Area	220 m²/g
USP Type	L14

HPLC Columns

HPLC Columns

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns