General Western Blot Reagents

General Western Blot Reagents
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Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
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Form | Liquid |
Product Type | Western Blot Enhancer |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane
Nitrocellulose membrane, with a 0.45 µm pore size, is suitable for proteins of a wide range of molecular weights.
Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ iBind™ Flex Solution Kit
Detection kit for use with iBind™ Flex Western System
No. of Reactions | 10 midi blots or 20 mini blots |
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Product Type | Flex Solution Kit |
For Use With (Equipment) | iBind Flex Western Device |
Membrane Compatibility | PVDF, Nitrocellulose |
Product Line | iBind™ |
Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Invitrogen™ XCell II™ Blot Module
Easy transfer of proteins or nucleic acids from mini-gels to membranes
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Thermo Scientific™ Pierce™ Western Blot Signal Enhancer
Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.
Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent
Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane
Product Type | ECL Western Blotting Detection Reagent |
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Storage Requirements | 18°C to 30°C |
For Use With (Application) | Routine and confirmatory Western blotting |
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | Nitrocellulose, PVDF |
Green Features | Less hazardous |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogen™ |